Melatonin Promotes Oligodendroglial Maturation of Injured White Matter in Neonatal Rats

نویسندگان

  • Paul Olivier
  • Romain H. Fontaine
  • Gauthier Loron
  • Juliette Van Steenwinckel
  • Valérie Biran
  • Véronique Massonneau
  • Angela Kaindl
  • Jeremie Dalous
  • Christiane Charriaut-Marlangue
  • Marie-Stéphane Aigrot
  • Julien Pansiot
  • Catherine Verney
  • Pierre Gressens
  • Olivier Baud
چکیده

OBJECTIVE To investigate the effects of melatonin treatment in a rat model of white matter damage (WMD) in the developing brain. Additionally, we aim to delineate the cellular mechanisms of melatonin effect on the oligodendroglial cell lineage. METHODS A unilateral ligation of the uterine artery in pregnant rat at the embryonic day 17 induces fetal hypoxia and subsequent growth restriction (GR) in neonatal pups. GR and control pups received a daily intra-peritoneal injection of melatonin from birth to post-natal day (P) 3. RESULTS Melatonin administration was associated with a dramatic decrease in microglial activation and astroglial reaction compared to untreated GR pups. At P14, melatonin prevented white matter myelination defects with an increased number of mature oligodendrocytes (APC-immunoreactive) in treated GR pups. Conversely, melatonin was not found to be associated with an increased density of total oligodendrocytes (Olig2-immunoreactive), suggesting that melatonin is able to promote oligodendrocyte maturation but not proliferation. These effects appear to be melatonin-receptor dependent and were reproduced in vitro. INTERPRETATION These data suggest that melatonin has a strong protective effect on developing damaged white matter through decreased microglial activation and oligodendroglial maturation leading to a normalization of the myelination process. Consequently, melatonin should be a considered as an effective neuroprotective candidate not only in perinatal brain damage but also in inflammatory and demyelinating diseases observed in adults.

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عنوان ژورنال:

دوره 4  شماره 

صفحات  -

تاریخ انتشار 2009